Composite
MaSp1-Cp19

Part:BBa_K5143003:Design

Designed by: Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-07-28)


MaSp1-Cp19k : a fused protein used as a bioglue


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 746
    Illegal PstI site found at 1049
    Illegal PstI site found at 1142
    Illegal PstI site found at 1148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 746
    Illegal PstI site found at 1049
    Illegal PstI site found at 1142
    Illegal PstI site found at 1148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 577
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 746
    Illegal PstI site found at 1049
    Illegal PstI site found at 1142
    Illegal PstI site found at 1148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 746
    Illegal PstI site found at 1049
    Illegal PstI site found at 1142
    Illegal PstI site found at 1148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for DNA synthesis and for Saccharomyces cerevisiae expression. GS linker for flexibility between the fused proteins.



Source

The source of the part will be its sequence which was retrieved from Yan et al., 2023.


References